AXR3 and SHY2 interact to regulate root hair development

Signal transduction of the plant hormone auxin centres on the regulation of the abundance of members of the Aux/IAA family of transcriptional regulators, of which there are 29 in Arabidopsis. Auxin can influence Aux/IAA abundance by promoting the transcription of Aux/IAA genes and by reducing the half-life of Aux/IAA proteins. Stabilising mutations, which render Aux/IAA proteins resistant to auxin-mediated degradation, confer a wide range of phenotypes consistent with disruptions in auxin response. Interestingly, similar mutations in different family members can confer opposite phenotypic effects. To understand the molecular basis for this functional specificity in the Aux/IAA family, we have studied a pair of Aux/IAAs, which have contrasting roles in root hair development. We have found that stabilising mutations in AXR3/IAA17 blocks root hair initiation and elongation, whereas similar mutations in SHY2/IAA3 result in early initiation of root hair development and prolonged hair elongation, giving longer root hairs. The phenotypes resulting from double mutant combinations, the transient induction of expression of the proteins, and the pattern of transcription of the cognate genes suggest that root hair initiation is controlled by the relative abundance of SHY2 and AXR3 in a cell. These results suggest a general model for auxin signalling in which the modulation of the relative abundance of different Aux/IAA proteins can determine which down-stream responses are induced

Mapping the Beta-Sheet Structure of the Yeast Prion Sup35 through Creation of Targeted Mutant Forms

Proteins with an aggregated form rich in beta-sheet structure are known as amyloids, of which a subset are infectious. These infectious proteins are known as prions and cause diseases including bovine spongiform encephalopathy (“Mad Cow” disease). Several prions have been identified in the baker’s yeast, Saccharomyces cerevisiae. One of the most well-studied yeast prions is the protein Sup35. To understand the fine protein structure of Sup35 better, we used PCR-based mutagenesis to introduce a lysine residue (a charged amino acid) at five defined places in the protein sequence of Sup35. We describe our process for creating these mutant versions and the results of DNA sequencing of each mutant version. The next step will be to assess prion formation and stability of clones with the correct sequences. Understanding the behavior of yeast prions has proven helpful in understanding human amyloid diseases and further studies on these yeast prions, including Sup35, will expand our knowledge further

Climate change impacts on water for irrigated horticulture in the Vale of Evesham. Final Report

This project has undertaken a scoping review and assessment of the impacts of climate change on irrigated horticulture in the Vale of Evesham, an area of intense irrigated production located within the Environment Agency’s Warwickshire Avon CAMS Catchment. The research was based on a combination of methodologies including desk-based review of published and grey literature, computer agroclimatic and water balance modelling, GIS mapping, meetings with key informants and a stakeholder workshop. Future climate datasets were derived from the latest UK Climate Impacts Programme (UKICIP02) climatology, using selected emission scenarios for the 2020s, 2050s and 2080s. These scenarios were then used to model and map the future agroclimatic conditions under which agriculture might operate and the consequent impacts on irrigation need (depths of water applied) and volumetric demand. This was complimented by a postal survey to abstractors and a stakeholder workshop, to identify, review and assess farmer adaptation options and responses. The key findings arising from the research, implications for water resource management and recommendations for further work are summarised below. Using a geographical information system (GIS), a series of agroclimate maps have been produced, for the baseline and selected UKCIP02 scenario. The maps show major changes in agroclimate within the catchment over the next 50 years. The driest agroclimate zones are currently located around Worcester, Evesham, Tewkesbury and Gloucester, corresponding to areas where horticultural production and irrigation demand are most concentrated. By the 2020s, all agroclimate zones are predicted to increase in aridity. By the 2050s the entire catchment is predicted to have a drier agroclimate than is currently experienced anywhere in the driest parts of the catchment. This will have major impacts on the pattern of land use and irrigation water demand. Cont/d

Priorities for sustainable turfgrass management: a research and industry perspective

This paper provides a brief review and assessment of the key environmental, regulatory and technical issues facing the turfgrass sector with specific reference to the European context. It considers the range of externalities or ‘drivers for change' facing the industry, and the challenges and opportunities available for promoting and achieving more sustainable turfgrass management within the sports, landscape and amenity sectors. The analysis confirms that there are a number of key areas where a concerted research and industrial effort is required. These include responding to the pressures from government demands for greater environmental regulation, the increasing pressure on natural resources (notably water, energy and land), the emerging role of turf management in supporting ecosystem services and enhancing biodiversity, the continued need to promote integrated pest management, and the looming challenges posed by a changing climate, and urgent need to adapt. Whilst many of these externalities appear to be risks to the sports turf industry, there will also be significant opportunities, for those where the labour, energy and agronomic costs are minimized and where the drive to adopt a multifunctional approach to sportsturf management is embraced

Porous squeeze-film flow

The squeeze-film flow of a thin layer of Newtonian fluid filling the gap between a flat impermeable surface moving under a prescribed constant load and a flat thin porous bed coating a stationary flat impermeable surface is considered. Unlike in the classical case of an impermeable bed, in which an infinite time is required for the two surfaces to touch, for a porous bed contact occurs in a finite contact time. Using a lubrication approximation an implicit expression for the fluid layer thickness and an explicit expression for the contact time are obtained and analysed. In addition, the fluid particle paths are calculated, and the penetration depths of fluid particles into the porous bed are determined. In particular, the behaviour in the asymptotic limit of small permeability, in which the contact time is large but finite, is investigated. Finally, the results are interpreted in the context of lubrication in the human knee joint, and some conclusions are drawn about the contact time of the cartilage-coated femoral condyles and tibial plateau and the penetration of nutrients into the cartilage

Squeeze-Film Flow in the Presence of a Thin Porous Bed, with Application to the Human Knee Joint

Motivated by the desire for a better understanding of the lubrication of the human knee joint, the squeeze-film flow of a thin layer of Newtonian fluid (representing the synovial fluid) filling the gap between a flat impermeable surface (representing the femoral condyles) and a flat thin porous bed (representing the articular cartilage) coating a stationary flat impermeable surface (representing the tibial plateau) is considered. As the impermeable surface approaches the porous bed under a prescribed constant load all of the fluid is squeezed out of the gap in a finite contact time. In the context of the knee, the size of this contact time suggests that when a person stands still for a short period of time their knees may be fluid lubricated, but that when they stand still for a longer period of time contact between the cartilage-coated surfaces may occur. The fluid particle paths are calculated, and the penetration depths of fluid particles into the porous bed are determined. In the context of the knee, these penetration depths provide a measure of how far into the cartilage nutrients are carried by the synovial fluid, and suggest that when a person stands still nutrients initially in the fluid layer penetrate only a relatively small distance into the cartilage. However, the model also suggests that the cumulative effect of repeated loading and unloading of the knees during physical activity such as walking or running may be sufficient to carry nutrients deep into the cartilage

International Travel and Its Impacts on Black/African American Identity Construction

How does going abroad impact Black/African Americans’ conceptualization of self? To assess the answer to this question I analyzed and reflected on mine and the international experiences of my participants, conducted thirteen interviews, and had participants answer survey questions. I argue that identity has two parts: your external and internal parts. The external identity I attributed to international experiences. My findings showed there are three impacts international travel has on Black/ African American identity constructions: the reinforcement, creation of something new, and added new dimension. There is little scholarship that studies the impact of international travel as it pertains to the diverse identity constructions within the Black community (Black vs African American). The impact of international travel on Black people’s identity construction is great, but especially for African Americans due to their unique identity constructions. I argue there is a divide between the impact of international travel on Black people (those who identify with Caribbean and or African roots) and African Americans (those with ancestors a part of the Transatlantic Slave Trade and with multi-generational history in the US). Black participants had international experiences that reaffirmed their identity constructions, while African Americans experienced identity fatigue, the unsettling challenge of their identity constructions. To better understand the modern Black/African American experience we must analyze the relationship between identity construction and external experiences. To do this we must consider African American’s unique identity constructions and its impacts on their modern experiences, international travel being one example

The economics of irrigating wheat in a humid climate - A study in the East of England

In the UK, wheat is the most important cultivated cereal, grown extensively as a rainfed crop. Irrigation of wheat has previously been considered uneconomic, but increases in world wheat prices and recent droughts have led to some farmers revising their views. Widespread adoption of wheat irrigation would have major implications for wheat production, the irrigation industry and water resources in regions that are already water scarce. This study investigated the financial viability of irrigating winter wheat grown on a sandy loam soil in the East of England. Long-term climate data (1961–2011) for Silsoe (Bedfordshire) was used to drive a biophysical crop model to assess irrigation water requirements and yield response. Modelling assumed a typical irrigation schedule to maximise yield and quality, and average reported wheat prices for 2007 to 2012. Irrigation costs were calculated assuming an overhead mobile hosereel–raingun system applying river water, abstracted either in summer and used directly, or abstracted in winter and stored in an on-farm reservoir. The results suggest that the yield benefit would justify supplemental irrigation by farmers who have unused irrigation equipment and unused summer water, although irrigation of higher-value field vegetable crops later in the season would normally take precedence – the Added Value of Water (AVW) usefully applied to milling winter wheat under these conditions ranged between 0.24 and 0.32 £ m−3. Investment in new irrigation schemes could also be marginally viable if unused summer river water was available for direct abstraction (AVW = 0.08 £ m−3). Investments in new farm reservoirs for irrigating wheat are currently not profitable (AVW = –0.23 £ m−3). Sensitivity analysis suggests that in the longer term, the expected increase in world wheat prices and the impacts of climate change are likely to make the financial benefits stronger, particularly in the drier catchments further east and on low moisture retentive soils, but competing demands for water would still make extensive wheat irrigation unlikely

Scalable Co-Evolution of Soft Robot Properties and Gaits

The field of soft robotics is very promising; applications in-clude urban search and rescue and covert surveillance, but these projects are not yet realized, partly because of the difficulties in soft robot shape and locomotion design. Be-cause of this, traditional design methods do not prove to be effective. This project attempts to come up with solu-tions to this soft robot design problem; utilizing a genetic algorithm, a computer simulation of Darwin’s “Survival of the Fittest,” this project attempts to make soft bodies move. This genetic algorithm evaluates each solution in simulation, and assigns each one a fitness based on distance travelled. Furthermore, this project implements a technique called co-evolution, which evolves two different things in lockstep, uti-lizing new found advancements in one to help bolster the other. This project evolves soft bodies’ physical properties, values that affect how they move, alongside locomotion tech-niques, the gaits defining their movement. Optimizations to this process are realized in the use of scalable soft meshes; this system starts on a simple mesh, and slowly increases its density, reducing the overall computation time

The Contribution of "Shared Education" to Catholic-Protestant Reconciliation in Northern Ireland: A Third Way?

Northern Ireland has achieved political stability and its devolved government is now tackling public policy issues neglected during periods of sectarian violence. Notwithstanding the prevailing political optimism, one legacy of the conflict is a deeply divided society. This is particularly manifest in the education system where around 90% of children attend either state (Controlled) schools (de facto Protestant) or Catholic (Maintained) schools, with integrated schools accounting for less than 6% of the school population. In an attempt to address this wasteful duplication of services, external funders have piloted an initiative entitled The Shared Education Programme (SEP) where schools working in cross-community partnerships deliver shared classes and activities in order to improve education outcomes. This paper attempts to: quantify the educational returns for pupils participating in the SEP; articulate the qualitative reconciliation benefits from the perspective of teachers, parents and pupils; and, locate the findings of the research in the ongoing policy debate about restructuring education provision in Northern Ireland at a time of budget retrenchment and declining school rolls